About combining the forward and reverse sequence
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28 days ago
ruddhida • 0

I have a sanger sequence of reverse and forward reads, but when i trimmed in geneious software it showed no contig found. This no contig found implies that there is no overlap of forward and reverse sequence. Does that mean there is an error in sanger sequencing or can we do anything about such reads.

16srRNA geneious • 300 views
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show the read pair here, it would be hard to comment on what one might see not knowing what the data is what has been done to it

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Reasonably certain geneious uses tools from BBMap suite to do some of these operations. You should merge the reads first before checking if they have contaminants.

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26 days ago

You can also try to play with one pair of sequences manually, since one of the sequences would have to be reverse complement to the other.

You can try pasting one sequence here and see if you can find any overlap.