Detection of bacterial SNP using regular PCR primers
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4 weeks ago
Audrey ▴ 50

Hi all,

I have two bacterial strains that only differ from several SNPs in their genomes. I am wondering if designing specific primer sequences containing the SNP mutation will be enough to differentiate the two strains using regular PCR?

I have heard of locked nucleic acid oligonucleotides but I would rather use regular PCR if possible.

Thank you in advance for you much appreciated help and advices!

Audrey

detection PCR SNP • 132 views
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I doubt sequences differing by only a single SNP allow you to distinguish by PCR alone. If you want to avoid sequencing, would restriction digests be an option for your SNPs of interest?

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