Entering edit mode
3.0 years ago
Sophie
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We are looking for a substrate that will start as dsDNA or RNA and that we can then monitor for its change to ssRNA/DNA through a fluorophore (or other measurable detection signal) during or at the end of the assay…which means the entire strand does not need to be ss at the end, but we do need a free end at the end of the reaction. We’ve been thinking about using “chaser” molecules that will disrupt the ability for the original dsDNA/RNA to reform after it is unwound but will not directly compete to break the substrate at room temperature when added to the same solution before adding enzyme. I’m hoping that your years of molecular biology experience can help us with some of our design questions.
I think you should ask this at a more suitable community that is more wetlab-orientated, such as a suitable SubReddit or ResearchGate. It is off-topic here in this bioinformatics community.
Ok thank you