Transcript enrichment in Illumina RNAseq
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17 days ago
compuTE ▴ 60

Hello,

I am wondering if someone could guide me or recommend me some reading material to understand what sort of enrichment (if any) can I expect from a paired-end Illumina TruSeq RNAseq sequencing. I mean, is there a 3' or 5' enrichment that I should be aware of? (as for example 10x does in their 5' or 3' library prep kits).

Also, I would love to start a discussion on how to test this -- For future reference and for different library preparation kits.

Thanks!

enrichment transcriptomics illumina rnaseq • 259 views
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RNAseq blog has several articles related to bias (LINK).

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Are you trying to understand the different library preparation methods that are typically used for bulk RNA-seq? This review paper by Griffith et al (2015) is a great place to get started.

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Thank you Friederike I'll check it out!

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