Do I need to assume differences in coverage profile when I compare the first reads from paired end data with single end read data?
1
0
Entering edit mode
16 days ago

I am considering feeding paired-end sequencing data into a bioinformatics workflow for coverage profiling which is actually adapted to single-end reads. So my plan is to use only the first reads as input.

My question now is whether I have to assume systematic differences in the resulting coverage (given identical read lengths), or whether I can expect that the coverage resulting from single-end reads and the first read from PE data are comparable in principle. Does anyone have experience with this?

Many thanks in advance!

single-end paired-end sequencing mapping coverage • 947 views
ADD COMMENT
1
Entering edit mode
16 days ago
GenoMax 100k

Unless a significantly different library preparation method was used the first read from a paired-end library should be equivalent to a library that was sequenced only from one end.

ADD COMMENT

Login before adding your answer.

Traffic: 1748 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6