Detecting mono-allelic expression in RNA-seq in human
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7 months ago
heskett ▴ 80

Hi all,

I have RNA-seq data on a haplotype-resolved cell line and I want to detect genes where there are more reads expressed from one chromosome allele than the other. First I counted informative reads, and then used the binomial distribution, analogous to a coin toss, to determine a p-value. The problem is that highly expressed genes always lead to a low p-value. Is there any way to add a parameter to the binomial distribution to make the tails "wider"? Or use a different distribution?

Thanks!

binomial allele_specific gene_expression rna-seq • 164 views
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