Transcript enrichment in Illumina RNAseq

Question

[compuTE]

Hello,

I am wondering if someone could guide me or recommend me some reading material to understand what sort of enrichment (if any) can I expect from a paired-end Illumina TruSeq RNAseq sequencing. I mean, is there a 3' or 5' enrichment that I should be aware of? (as for example 10x does in their 5' or 3' library prep kits).

Also, I would love to start a discussion on how to test this -- For future reference and for different library preparation kits.

Thanks!

[GenoMax]

RNAseq blog has several articles related to bias (LINK).

[Friederike]

Are you trying to understand the different library preparation methods that are typically used for bulk RNA-seq? This review paper by Griffith et al (2015) is a great place to get started.

[compuTE]

Thank you Friederike I'll check it out!