I need to get the transcription start site for several genes. However, the ncbiRefSeq file from the UCSC browser gives more than one TSS value for one gene (because some genes have several transcripts). If I need to choose one TSS value, how should I do this? How should I identify the more "common" TSS for a gene? Or if there's another approach to choose one TSS value, please comment below!
Thank you very much!
I haven got to using it yet, but maybe you could use the NCBI-EMBL MANE track. It provides a unique representative transcript making use of both NCBI + ensembl knowledge (it's a work in progress so maybe all genes haven't been covered yet).
I see! I checked it out:) thank you so much for your suggestion!!
Thank you for the reference!
Does TSS = first exon?