Hello everyone, I have a problem when using FeatureCounts to assign my Nanopore. When using it with a Bam file i always have 0 total alignment recognized, and so i have no successfully assigned alignment. I think the problem is that FeatureCounts refuse to read my bam, or cannot read it for some reason (when i use it for another Bam resulting from another Run i have no problem with it).
Can you help me please ?
Ps : I've aligned with minimap2, sorted and indexed, and I've also checked the gtf chromosome format.