Entering edit mode
3.0 years ago
madziapio
•
0
Dear All,
I have trouble with amplification of my insert from cDNA library. I want to amplificate my gene of interest and then transfer it to my vector. My primers's Tm is around (76 vs 70 degree). I use 0,5 ng of the library. I don't see any product on gel. Any ideas why?
Thank you
