Why do I keep encountering the following errors whenever I run the naccess program to calculate the interface area of a protein with multiple chains?
Entering edit mode
5 months ago
Cm • 0

I want to calculate the interface area of several proteins using pdb files consisting of two chains, for instance, 1P9U_AG.pdb, having the chains A and G. However, every time I use the naccess to do so from the terminal, it gives output as follows

Program received signal SIGSEGV: Segmentation fault - invalid memory reference.

Backtrace for this error:

0 0x7fba08e69d3a

1 0x7fba08e68ed5

2 0x7fba08b4e20f

3 0x7fba090c3155

4 0x55835f8ccd09

5 0x55835f8cd084

6 0x7fba08b2f0b2

7 0x55835f8c821d

8 0xffffffffffffffff

Segmentation fault (core dumped)

Though this does create the expected .rsa, .asa, and log files, they either don't have any relevant data when I open them or a message like the one below pops up

"Unexpected error: Invalid byte sequence in conversion input."

The program works perfectly fine with pdb files comprising of individual chains though. I would be really grateful if someone offers a solution to resolve this issue.

A pic of what appears when I try to run the naccess program through a shell script a view of one of the .rsa files that get created

Naccess Interface-area PDB PPI • 278 views

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