Tool for list protein mutation sites from WT/variants sequences

Question

[gamamiguelangel]

Hello!

Anyone can help me please? Do you know a tool for map the mutations sites given a WT protein sequence and proteins variants?

For example:

wt.fasta (1 seq in fasta format):

wt_protein_example MAAAAAAAAAA ...

variants.fasta (in multifasta file format)

Variant_1 MAAAAAAAAAA ...

Variant_2 MATAAAAAAAA ...

Variant_3 MAAAPAAAARG ...

And for each protein sequence in variants.fasta file get a table (.CSV, etc) of mutations like:

Properly, i have 1 WT seq of TEM-1 b-lactamase and ~300,000 protein variants generated mi error prone PCR