Compatilibity between human37 and human38 gtf

Question

[asumani]

Hi,

I aim to make miRNA:target prediction and later I will look for the target gene expression levels from the available count matrix. Count matrix is published in which authors used Ensembl GRCh37v75 transciptome for alignment.

For target prediction, I have to obtain 3UTR fasta file first. Should I also use Ensembl GRCh37v75 gtf to obtain 3UTR fasta? Is it a problem if I use Ensembl GRCh38 gtf?

[WouterDeCoster]

Is it a problem if I use Ensembl GRCh38 gtf

Yes. The coordinates are not guaranteed to match.