Functional annotation
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3.0 years ago

I have performed functional annotation of Rice chromosomes 9 and 10 from Galaxy tools and got results in the form of GFF. I have tried all the tools to find the function of genes associated with gene IDs but couldn't find any such tools. Kindly recommend me some tools or databases to find functions from these gene IDs.

Annotation • 698 views
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did you perform structural or functional annotation? aka, did you predict the location & structure of genes or did you had the genes and you went looking for the function of those genes?

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I have performed functional annotation on the DNA sequences of chromosome 9 and 10 by simply uploading the Fasta file in Galaxy tool (Augustus) and I got the results in GFF format and there is all the information in the file like start and stop codons and gene IDs but I am interested in finding the functions from these gene IDs. So, kindly suggest me some tools or databases from which I can retrieve functions from these gene IDs.

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OK, then you did structural annotation (not functional!) . Yes that indeed outputs things like GTF files and such ...

next to do is looking for functions as you mention. What kind of tools did you use already? what else have you considered doing?

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