Hello Biostars community,
I am trying to create a KO of my target gene. After editing my target gene in CRISPOR, I used the cDNA without my KO region into expasy translate to find out the possible outcome after translation.
Here we find the 6 different open reading frames in the forward and the reverse direction. One of my reading frames, is very similar to the target WT protein, which I also confirmed through BLAST.
How to identify if this particular ORF will be biologically relevant, meaning to ask if this ORF will indeed be detected as a coding sequence and translated by a eukaryotic cell system? Any tools available to check the likelihood of which ORF will be detected as the coding sequence region?
Thanks in advance!