Hi fellow biologists, the fragment length distribution of my ATAC-seq data has no peak beyond ~100bp. So basically no peak near nucleosomes. However, the downstream analyses of the data make perfect sense (right motifs show up, right genes nearby etc after differential accessibility analysis).
How do I explain this? How big a deal is the fragment distribution in terms of QC of the data? All other QC factors are good (no of reads, base calling quality, GC content, % mapped reads etc).