I would like to perform a t-test to evaluate the gene expression level between the mutation and normal groups. Can anyone prefer a suitable package and guidelines?
What type of analysis is this?
A statistical student's t-test to compare gene expression levels between normal and mutation tissue samples.
You can't publish with a t-test result, it's just not a smart enough way to handle the math involved.
Do what everyone does. Run DESeq2 or EdgeR in R.
THIS! You need to go back and learn about what type of distribution is used for a t-test. Then start understanding the distributions that gene expression data follows. This will give you a quick insight as to why you cannot use a t-test, and why the tools swbarnes2 posted about are needed.
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