T-test analysis for gene expression comparison
1
0
Entering edit mode
5 weeks ago
Nithya ▴ 10

Hi there, I would like to perform a t-test to evaluate the gene expression level between the mutation and normal groups. Can anyone prefer a suitable package and guidelines?

RNA-seq • 265 views
ADD COMMENT
0
Entering edit mode

What type of analysis is this?

ADD REPLY
0
Entering edit mode

A statistical student's t-test to compare gene expression levels between normal and mutation tissue samples.

ADD REPLY
0
Entering edit mode
5 weeks ago

You can't publish with a t-test result, it's just not a smart enough way to handle the math involved.

Do what everyone does. Run DESeq2 or EdgeR in R.

ADD COMMENT
1
Entering edit mode

THIS! You need to go back and learn about what type of distribution is used for a t-test. Then start understanding the distributions that gene expression data follows. This will give you a quick insight as to why you cannot use a t-test, and why the tools swbarnes2 posted about are needed.

ADD REPLY

Login before adding your answer.

Traffic: 1711 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6