Where can I find the rRNA sequence of E.coli?

Question

[Inayat]

I have tried a lot to search the complete rRNA sequence of E.coli, but I am unable to find it out. However, what I am getting is the sequence for the 16S only. Please, if you can share the link, your help is appreciated

[antonioggsousa]

There are several ribosomal RNAs, 5S, 16S and 23S rRNAs. To find them search or blast across the E. coli genome, e.g., in NCBI: https://www.ncbi.nlm.nih.gov/assembly/GCF_000005845.2

Answer 1

[Michael]

Hi, may I first refer you to the Wikipedia article on ribosomal RNA: https://en.wikipedia.org/wiki/Ribosomal_RNA

This will explain the following facts in detail

• there is no singular "the complete rRNA" sequence
• instead there are three rRNA sequence species: 16S, 5S, and 23S
• these, together with proteins form the two ribosomal subunits: SSU and LSU
• multiple genes may encode the 3 RNA species

In addition there are many different e. coli strains. Here is an example query for the 5S gene in e. coli K12 substr. MG1655 Here is the corresponding Refseq sequence: https://www.ncbi.nlm.nih.gov/nuccore/NR_103249.1

Here is a query for all E. coli 5S rRNA sequences from RefSeq to enter in NCBI nucleotide search or use with Entrez e-utils:

"Escherichia coli"[organism] AND "5S ribosomal RNA" AND srcdb_refseq[PROP] 

[Inayat]

Thank you for the valuable information