clustalw warning: Bisectingkmeans(): Can't split cluster no. xx
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11 months ago
seok1213neo ▴ 20

im trying to run clustalw to align quite a many sequences.

all i have commanded is this

clustalo -i Bacteria.fsa -o ./Bacteria.msf

but i am keep on getting this warning sign

WARNING: BisectingKmeans(): Can't split cluster no. 5 which has 104 objects any further. Hope it's not too big and doesn't slow things down.
WARNING: BisectingKmeans(): Can't split cluster no. 143 which has 178 objects any further. Hope it's not too big and doesn't slow things down.
WARNING: BisectingKmeans(): Can't split cluster no. 223 which has 187 objects any further. Hope it's not too big and doesn't slow things down.
WARNING: BisectingKmeans(): Can't split cluster no. 163 which has 110 objects any further. Hope it's not too big and doesn't slow things down.
WARNING: BisectingKmeans(): Can't split cluster no. 10 which has 195 objects any further. Hope it's not too big and doesn't slow things down.
WARNING: BisectingKmeans(): Can't split cluster no. 76 which has 540 objects any further. Hope it's not too big and doesn't slow things down.
WARNING: BisectingKmeans(): Can't split cluster no. 1 which has 269 objects any further. Hope it's not too big and doesn't slow things down.
WARNING: BisectingKmeans(): Can't split cluster no. 365 which has 124 objects any further. Hope it's not too big and doesn't slow things down.
WARNING: BisectingKmeans(): Can't split cluster no. 304 which has 110 objects any further. Hope it's not too big and doesn't slow things down.
WARNING: BisectingKmeans(): Can't split cluster no. 370 which has 137 objects any further. Hope it's not too big and doesn't slow things down.
WARNING: BisectingKmeans(): Can't split cluster no. 419 which has 189 objects any further. Hope it's not too big and doesn't slow things down.

how should i fix this?

please help me thank you!

bisectingkmeans clustalomega clustalw clustalo • 487 views
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Now I also encounter this problem, may I ask, have you solved this problem? If so, how did you do it

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