Is removing of the one trailing nucleotide in Illumina reads necessary step of data preprocessing
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14 months ago
Denis ▴ 230

I've read information that if Illumina reads lenght looks like 101bp,151bp,251bp, 301bp, etc., someone have to remove the last (one trailing) nucleotide from 3' end of the read before any preprocessing steps. I'm wondering if it's indeed necessary? Can you help me please?

Illumina Preprocessing • 357 views
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14 months ago
GenoMax 118k

No you don't need to remove that base. If you are the worrying kind you could do that if you are doing de novo assembly work. Aligners will soft clip that base if it does not align with a reference.

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Thank you very much!

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