We have sequenced a chloroplast DNA from potato and assembled the reads by SPAdes assembler. After assembly, we got 3 contigs.
Now we want to perform the following steps:
- Generate a single DNA sequence from these 3 contigs.
Q. Would you please suggest any pipeline or tool or method to do this?
- Then we want to use this single DNA sequence for annotation, where we should get .gff, .gb, etc files.
Q. Which tool we should use for this purpose?
Thanks in advance.