Sequence Read Archive (SRA) submission ERROR
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6 months ago

Hi every one, i have this errror when i try to submit a sample

Your table upload failed because multiple BioSamples cannot have identical attributes. You should have one BioSample for each specimen, and each of your BioSamples must have differentiating information (excluding sample name, title, bioproject accession and description). This check was implemented to encourage submitters to include distinguishing information in their samples. If the distinguishing information is in the sample name, title or description, please recode it into an appropriate attribute, either one of the predefined attributes or a custom attribute you define. If it is necessary to represent true biological replicates as separate BioSamples, you might add an 'aliquot' or 'replicate' attribute, e.g., 'replicate = biological replicate 1', as appropriate. Note that multiple assay types, e.g., RNA-seq and ChIP-seq data may reference the same BioSample if appropriate.

And, this is my file list

LIVER_H1_1_1
LIVER_H1_1_2
LIVER_H1_2_1
LIVER_H1_2_2
LIVER_H1_4_1
LIVER_H1_4_2
LIVER_H3_1_1
LIVER_H3_1_2
LIVER_H282_1
LIVER_H282_2
LIVER_H303_1
LIVER_H303_2
LUNG_P1_5_1
LUNG_P1_5_2
LUNG_P1_6_1
LUNG_P1_6_2
LUNG_P1_7_1
LUNG_P1_7_2
LUNG_P2_2_1
LUNG_P2_2_2
LUNG_P284_1
LUNG_P284_2
LUNG_P374_1
LUNG_P374_2


the name of the samples is because they are paired end, for example LUNG_P374_1 and LUNG_P374_2 are the same samples, but one is paired end 5--3 and the other is 3--5 , also in the rest of the cells to fill, i have the same information in:

Organism, strain ,isolate, collection date, collected by, collection date, geo location, host , isolation source, lattiude and longitude.

i would appreciate any help, thank you!

submission rnaseq sra • 400 views
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It may be best to work with SRA support to address this since they can see your actual submission and will guide you on how to fix it.

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thank you! i have already contected the support. I will post the solution, as soon as possible.

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Did you ever figure out this solution? Thank you