I have a question on tools for building a phylogenetic tree. I have an alignment file of 60 strains from Roary tool (.aln file). And what I want to do is draw a phylogenetic tree based on this (aln file), so I tried FastTree, but it provokes an error saying, "This alignment consists of closely-related and very-long sequences." and it results in a blank nwk file. I also treid EMBL phylogenetic tree webtool, but it also says my file is too big to process.
Does anyone recommend any webtool or local tool I can build the tree??
Thank you in advance.