How to generate GTF file from BLAST output?
2
0
Entering edit mode
5 weeks ago

Hello All, How to create a GTF file, I have only sequence level of information available (fasta format)

Note: I am working on mitochondrial genome, no GTF file available in any repository

Regards Mathavan M

GTF • 293 views
0
Entering edit mode

A GTF file of what?

In general you question is lacking some crucial bits of information to get meaningful answers/support here.

0
Entering edit mode

I am working on bovine genome

0
Entering edit mode

Since the title of the question refers to converting a BLAST output to GTF here is one representative tool (LINK). You will need to see if it works for your purpose. There are others but YMMV.

3
Entering edit mode
5 weeks ago

The Ensembl GTFs contain the mitochondrial genes.

0
Entering edit mode

Mam I working on Bubalus bubalis (water buffalo)

2
Entering edit mode
5 weeks ago

I don't think this is what the OP wants, but to answer the actual question:

Probably the easiest way to get BLAST output as a GTF file is to use commandline BLAST, and to set the outfmt to 17 (SAM), and then convert the SAM output to gff. And the easiest way to do that might be sam2bed and then bed2gff from bedops.