Should do Seurat NormalizeData after Cellranger aggr?
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2.9 years ago
hq.huang11.6 ▴ 10

Hello, I feel confused about the workflow of scRNA

If I do the cellranger aggr, which normalizes the sequencing depth. Should I still need to do the Seurat NormalizeData? I think NormalizeData also wants to remove the effect of the sequencing depth. Can I just log the data and do the next analysis?

Thank you for any comment!!!

scRNA • 646 views
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