Hi ! We have started work on proteome analysis and we are using label free quantitative with Mass spec. From the results I know I need to compare the Lfq intensity values, only I can not fully understand the process and how they came to these values. I thought the way to do that is by calculating the intensity of the protein's peptides and then dividing by the number of its theoretical peptides. According to the process described there is no such step. Can anyone explain to me?