Hello every one I'm trying to obtain differentially expressed genes in galaxy using Deseq2. But unfortunately, I get the following error. I need to mention that my data is pair end and the stringtie results are in a list.
Fatal error: An undefined error occurred, please check your input carefully and contact your administrator. Tool generated the following standard error: estimating size factors Error in estimateSizeFactorsForMatrix(counts(object), locfunc = locfunc, : every gene contains at least one zero, cannot compute log geometric means Calls: DESeq ... estimateSizeFactors -> .local -> estimateSizeFactorsForMatrix