Entering edit mode
2.8 years ago
almogangel
•
0
Hi everyone,
I have two unique library structures that I am trying to demultiplex using bcl2fastq2.
The first have 8bp long barcode before the R1 promoter which is 33bp long: 5'-adapter-barcode-R1_promoter-cDNA ...
The second have 7bp long barcode after the R1 promoter which is 29bp long. 5'-adapter-R1_promoter-barcode-cDNA ...
Both of them are paired-end that do not have a barcode for the second read.
looking at old posts I noticed that I need to set "--use-bases-mask", but I am not completely sure how.
In addition, do I also need two separate sample sheets?
P.S We used the NovaSeq 6000, so the barcode needs to be written as a reverse complement?
Thanks!
