I have some Indrop scRNA-seq
The number of genes per cell and number of read seems like this
Here what I should put to get a good Seurat object?
I have tried this but I think I am missing some cell types
pbmc <- subset(pbmc, subset = nFeature_RNA 1000 & nFeature_RNA < 5000 & percent.mt < 25)
Please look at these plots to give me an intuition which numbers give better results