Hello, I am completely new to Sequencing and programming (and I am blonde) - so please bear with me.
I already saw that there are some questions about it, but I could not really understand/deduce what I have to do now. So, I have the task to recreate some figures of a paper with RStudio. I choose the single-cell RNA-Seq results from this paper: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8077299/ I already downloaded all SRA files via the SRAtoolkit and I am already converting them in FastQ files with the split-3 option. And I know I have to check them with FastQC afterwards. But, there are for each sample two SRA runs (this sample for example: https://www.ncbi.nlm.nih.gov/sra/SRX8998846[accn] ) Why are there 2 SRA files, which will result in ultematively in 4 FastQ files for one sample? I have read somewhere "technical duplicates", but there is also this huge difference in size (6.9 GB and 18.3 GB) and if I am clicking on the runs to get more informations I get lost.
Can someone please explain to me why there are 2 SRA files are and how I should proceed with them?