I am currently performing a metagenomic analysis on transcriptomics data. But I'm not sure what strategy to adopt in relation to the assembly.
In fact, the transcriptomics data are from three biological replicates per sample. I am wondering whether I should concatenate the replicates during assembly or not.
And what would be the impact if I wanted to analyse my results with EdgeR. As a newbie in bioinformatics, your help would be appreciated.