Entering edit mode
2.8 years ago
rashmirao962
▴
10
What is the linear region and co-factor ? How are they related?
What is the linear region and co-factor ? How are they related?
Hi, I think that the function documentation explains it quite well, and in a simple way.
Key points:
Kevin
Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
@Kevin Blighe , thank you for the answer. My understanding is that, (1)The linear region is the area under the curve,not the area of the bar with the highest frequency. (2)with increase in co-factor the width of the linear region decreases. (3)The purpose of the transformation is to scale down the data and achieve a more Gaussian distribution of the data set.However, in the PBMC dataset(http://imlspenticton.uzh.ch/robinson_lab/cytofWorkflow/).Upon plotting the histogram for different co factor values(2,5,10)for the arcsinh transformed expression matrix ,I noticed that at at co-factor value of 2 the distribution is more Gaussian than at co-factor value of 5. (4)Wouldn't 2 be more suitable in this case?
Co-factor 10
Co-factor 5
Co-factor 2
Thank you,
Rashmi