How to know the positions of contigs in a reference genome
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4 months ago
Ashi ▴ 10

Hi I am quite new to Bioinformatics and this site has always helped me. I have generated contigs (FASTA file) of E coli from short read assemblers like PaKman, Minia. I want to know the positions of these contigs in the reference genome now. Is there any tool to do that? I have seen the earlier posts here saying that blast can give the positions but I could not get the command line to do so. I know quast gives you the position of the misaligned contig positions but I want to know the positions of the all the contigs that I have got from the short read assembler.

Thanks for all the help.

mapping positions alignment reference genome contigs • 227 views
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4 months ago
GenoMax 107k

You can simply align the contigs to E. coli genome. minimap2 or lastz would be good options.

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3 months ago
Jordi • 0

Another option that works well for aligning FASTA files is MAFFT (https://mafft.cbrc.jp/alignment/software/).

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