I have the following two genes with expression taken from single-cell RNAseq:
I am conducting pairwise comparison between sample (S1, S2...) on those data, using t.test. The data has been normalized using Monocle3 normalization function.
My question is whether this is a correct approach? If not what are the better statistical test to use?
And also, if it's also acceptable to perform statistical test for raw count? And the need of multiple comparison test like Bonferroni, FDR?