Entering edit mode
2.8 years ago
isha.lily20
▴
10
Hello Researchers , 1) can any one tell me how to do a reciprocal blast ?
2)a) how to identify conserved orthologous genes and annotation of specific sequences ? and than 2)b) how to map specific sequences on conserved orthologous genes ?
i tried taking 3 protein sequences of different species and did normal protein blast with specific e value 1*10^(-6)
but i dont kno what is reciprocal blast , how to find evolutionary conserved orthologous genes ?
thnk u ....
If a
Reciprocal Best Hit
(RBH
) search is what you want to do, try theeasy-rbh
module fromMMseqs2
.As for your second (and maybe third) questions, dig around in this book perhaps?
Hi Ram, i tried the same as mentioned exported 3 protein sequences of different species just did normal protein blast
Please google "reciprocal blast" - there are Biostars posts that address the topic. Always search online and try implementing a reliable solution you find on an online forum/paper.