Splitting predicted transcripts according to ORF coordinates
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2.8 years ago
Buffo ★ 2.4k

I have the following situation, 3 ORFs contained in a single transcript (assembled using StringTie). I have tried different custom parameters for the transcript assembly to reduce this "redundancy" but nothing worked for some regions. I don't want to reinvent the wheel, so before writing a script (I have thousands of similar cases) to split those transcripts using the ORF coordinates, I was wondering if you have any suggestions, is there any tool to manage this?

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transcript ORF RNAseq • 415 views
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