Entering edit mode
2.8 years ago
Apex92
▴
280
Hi everyone - I have single-cell RNA sequencing (single-ended) - I used --outFilterMultimapNmax 1
as the alignment option with STAR to report only the unique mapping reads in BAM files. I want to generate read count tables using featureCounts
but I want to divide read counts that map to two overlapping features - is it proper to use --fraction
, -M
and -O
options of the featureCounts for this purpose?
Thanks