Entering edit mode
20 months ago
A_heath ▴ 120
I would like to have some help please to understand the genotype of a genetically modified bacterial strain (E. coli BL21 DE3) : F- ompT hsdSB (rB-mB-) gal dcm rne131 (DE3)
F- is for cells lacking the fertility factor but I don't have the other ones.
Thanks for your much appreciated help!
Please find the details here and here.
Thank you so much for this detailed genotype!
I have an additional question as I still identify lon proteases within the genome of BL21 DE3. Do you have any idea how is that possible as it is supposed to lack lon gene ?
How did you identify lon proteases?
Did you sequence the BL21 DE3 from your lab or you are using the database sequence?
On the genome of E. coli BL21 DE3 available on the NCBI database (CP001509.3), there is still at least one Lon protease (427,922 - 430,276 bp) and an additional one at: 1,021,628 - 1,023,388 bp so it doesn't make sense ... Thanks again for your help MSRS!
The IS186 at positions (426481..427823) of CP001509.3 play a role in the inactivation of lon protease deficiency. details here
So much more understandable now... Thank you very much for all the help provided, it's greatly appreciated! Have a nice day,