I don't know if this is an appropiate question here, but I am lost. Is here someone with RStudio and scRNA-Seq experience, who is willing to help me with my project? Maybe talking via Discord? (I am living in the time zone Europe/Berlin.)
I took this https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8077299/ and need to re-do the analysis of the scRNA-Seq parts, but after quite a few hours I got a complete different UMAP (there is no similarity between the paper and mine) and the search for marker genes, which should give me markers for the enterocytes, stem cells, etc,- resulted surprisingly in cancer cells. Furthermore, I honestly do not know how to proceed with the TAP-count matrix.
I asked the authors for their script, but I am not sure if they are going to help me. That's why I am asking here.