Entering edit mode
3.2 years ago
bioAddict
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0
Hi Everyone,
I assembled RNAseq reads for my viral metagenomics study. But after the analysis of the contigs yielded with viralverify and viralcomplete, I noticed that some contigs hit the same virus species. Could you please explain me how I should handle this contigs in order construct an index with bowtie2, to map the reads and compute the coverage of each virus specie.
Thanks in advance,
Hi,
Maybe I do not understand but, why this should be a problem?
I'm newbie in metagenomics. So I do not really understand also. That's why I asked the question. Could you please help me?
You can have different contigs mapping to the same species hence, each of these contigs will contribute to the final coverage of that specie