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3.2 years ago
pfchuckran
▴
30
I am looking to map soil metatranscriptomes against a set of MAGs I have assembled and I was wondering if it would be best practice to either: 1) map reads from my metatranscriptomes against gene calls from MAGs; 2) map against the contigs used to assemble the mags (prior to binning); 3) de novo assemble metatranscriptomes and then align to the metagenomic bins; 4) something else entirely. Any insights would be much appreciated - still very new to this.
Thanks
Hi,
Do you know now how to map the transcriptomes reads to the MAGs? and then calculate the transcript counts or even the specific genes?
Thanks!