"Illegal instruction" error when I use the latest STARsolo to analysis 10X genomics data
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4 days ago
whaiyu06 ▴ 70

I'm writing to ask for your help. I use the latest STARsolo to analysis the 10x genomics data(Single Cell 3' v3), and exit with the "Illegal instruction" error .But I can't pick out which parameter(s) is inappropriate. The command I used as follows:

STAR --runMode alignReads \
--runThreadN 16 --genomeDir $refgenome \ 
--readFilesIn B10X-5_S25_L004_R2_001.fastq B10X-5_S25_L004_R1_001.fastq \
--soloType CB_UMI_Simple \
--soloFeatures GeneFull \
--soloUMIlen 12 \
--soloCBwhitelist $whitelist \
--clipAdapterType CellRanger4 \
--outFilterScoreMin 30 \
--soloCBmatchWLtype 1MM_multi_Nbase_pseudocounts \
--soloUMIfiltering MultiGeneUMI_CR \
--soloUMIdedup 1MM_CR \
--soloCellFilter EmptyDrops_CR \
--outFileNamePrefix ./B10X-5

Thanks for your suggestions.

statsolo 10x • 332 views
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I ran the code without --clipAdapterType CellRanger4 and added the --soloBarcodeReadLength 0 , then the output is correct.However, it doesn't work when only added the --soloBarcodeReadLength 0. So the issue is the --clipAdapterType CellRanger4 , and I haven't settled.

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