just wondering if I have a list of all differentially expressed genes in a group of patients = eczemagenelist and want to see if a separate list of genes= mygenelist is enriched in the eczemagenelist. does it make sense if I divide the DEG in eczemagenelist into those with increased or those with decreased expression and compare mygenelist with these by using metascape?
Or is it not meaningful ie graphs will obviously be different for each group so is it a good way to understand the enrichments or is it too biased to select out the increased DEG from the decreased DEG ones.?
I already looked at the entire undivided list of eczemagenelist with mygenelist and input these into metascape.