Functional annotation by blast

0

Entering edit mode

2.7 years ago

pooryamb • 0

Dear all,

I want to annotate 11,000 genes with Blast2GO. For this, I have to run Blast for all of the genes (on a standalone server). I wondered whether I might lose anything if I blast my genes against TrEMBL (rather than nr). I feel like the genes in NR but not in TrEMBL do not have functional annotation, so it is useless to blast against them. But I am not sure.

I really appreciate any help you can provide.

trembl Blast nr Functional_annotation Blast2GO • 814 views

ADD COMMENT • link 2.7 years ago by pooryamb • 0

2

Entering edit mode

TrEMBL also contains computationally translated genes. If you want curated genes then you should look at UniProt of RefSeq proteins.

ADD REPLY • link 2.7 years ago by GenoMax 141k

0

Entering edit mode

Thanks for your reply. I think for some of the genes that are in TrEMBL, automatic electronic functional annotation is available. So, I guess if I blast against TrEMBL (rather than UniProt), I will get some new hits. But, I am not sure whether the genes in nr but not in TrEMBL have any automatic electronic functional annotation or not?

ADD REPLY • link 2.7 years ago by pooryamb • 0

Login before adding your answer.