Hello,
I have sent a few total RNA samples for RNA-seq (100 M reads, 100PE, rRNA depletion, DNBseq). However, the bioanalyzer results came back as unqualified because of RNA degradation and a RIN of 2.5-2.8. Is there any way I can proceed with the library preparation, or it's impossible that the sequencing will produce sufficient data?
Thank you very much for your time :)
The problem is that these samples include controls. And, the purpose of the sequencing was to analyze the whole transcriptome of total RNA extracted from very rare and "hard to come across" samples, no degradation or sonication was carried out after extraction. Of course, the sequencing company's advice is not to proceed with the sequencing, but I don't have other samples to send and I think in this case my whole project would be terminated.
Thank you so much for sharing your opinion, I appreciate it.