I performed the following steps with my data.
- Performed digital normalization on the individual 205 samples --- success
- removed the host (e.g. human) and adapter sequences from the individual samples --- success
- concatenated the samples into groups ---success
- performed the assembly with metaspades and megahit --- success
Tried to perform binning step but it gives the following error:
ERROR [mem_sam_pe] paired reads have different names: "A00534:36:HCHN3DRXX:2:1101:7437:1031", "A00534:36:HCHN3DRXX:2:1101:13693:1031"
Any idea how to fix this issue? Could it be digital normalization messing up the read names?
Would really appreciate your help!