Demultiplex Quality

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2.7 years ago

sx2263 • 0

Hi all,

I'm working on a project that requires to demultiplex the sequencing data twice (for example I have 400 samples, the first time I will demultiplex to 4x100 samples, and the second time I will demultiplex to individual file). I'm wondering if I should just demultiplex once and avoid any potential loss of reads? Will the quality of the data be lower if I demultiplex twice?

Thank you!

sequence demultiplex illumina • 578 views

ADD COMMENT • link updated 2.7 years ago by GenoMax 141k • written 2.7 years ago by sx2263 • 0

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Entering edit mode

I'm wondering if I should just demultiplex once and avoid any potential loss of reads? Will the quality of the data be lower if I demultiplex twice?

There is not enough information in your post as it stands. What are you demultiplexing using? Internal barcodes/Illumina indexes? Why should there be any potential loss of reads? Why should the quality be lower (assuming you are referring to quality scores of each base)?

ADD REPLY • link 2.7 years ago by GenoMax 141k

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