I have obtained the output file from QiaSeq GeneGlobe containing UMI counts. The description of quantification procedure is given here : https://www.qiagen.com/us/resources/resourcedetail?id=bea2dcfa-0a5c-47c5-afd8-8b0fe90a471a&lang=en
Subset of the data in the output excel file from 'miR_piRNA' sheet is given below
I'm not exactly sure why there are both UMI counts and read counts in the output file.
My assumption is that the READs columns are the initial counts obtained and then after collapsing the duplicate UMIs, the counts are given in UMIs columns. Is this correct ?
If so, then for further analysis - i.e. DE analysis etc, only the UMI columns are to be used ? Is there any use of the READs columns ?