Entering edit mode
3.2 years ago
Alexander
▴
220
A colleague of mine have got the following distribution of GC-content for RNAseq.
How to interpret bimodal distribution of GC-content for RNAseq ? Does it mean some contamination ? Is there any method to remedy ?
A broad peak usually indicates contamination. To confirm that, you can run fastQ screen.
Start checking for contamination with rRNA.