How to interpret bimodal distribution of GC-content for RNAseq and can it be remedied ?
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3.2 years ago
Alexander ▴ 220

A colleague of mine have got the following distribution of GC-content for RNAseq.

How to interpret bimodal distribution of GC-content for RNAseq ? Does it mean some contamination ? Is there any method to remedy ?

Figure

RNA-Seq GCcontent FastQC RNAseq • 1.6k views
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A broad peak usually indicates contamination. To confirm that, you can run fastQ screen.

FastQ screen allows you to search a large sequence dataset against a panel of different genomes to determine from where the sequences in your data originate.

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Start checking for contamination with rRNA.

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