Question: Average Width Of H3K4Me3 Peaks
1
gravatar for aditi.qamra
7.0 years ago by
aditi.qamra270
Toronto
aditi.qamra270 wrote:

Hi,

I used CCAT to call peaks for the H3k4me3 mark in my samples and the average peak width I noticed is ~3kb

I wanted to get a sense check on these nos. ? Usually in cancer samples, is that the sort of profile that is seen for H3k4me3. I tried looking this up in literature but I dint get enough data pts and the few that I did showed an average profile of 1.5kb.

Does anybody have any references on this ?

Thanks !

chipseq peak-calling • 3.0k views
ADD COMMENTlink modified 7.0 years ago by Sukhi Singh10k • written 7.0 years ago by aditi.qamra270
1
gravatar for Sukhi Singh
7.0 years ago by
Sukhi Singh10k
Netherlands
Sukhi Singh10k wrote:

From H3K4me3 in mESC (mouse embryonic stem cells), what I know is that the promoters which have the tri-methylated nuclesomes at active. This width, I plot as an average profile in and around +/-3KB of TSS, which is sufficient to answer. Its hard to say the exact width, but I would assume it to be a max of 3KB so it might be fine.

As an example, I took this screenshot from the cistrome webpage. Its obvious that the average width would be around 2-3KB but I am not sure if that be different in the cancer cell line.

TSS H3K4me3

ADD COMMENTlink written 7.0 years ago by Sukhi Singh10k

Hi Sukhdeep.. Thanks for this.. This gives me some consolation. The peaks im looking at are infact active promoter regions and some of my peaks even go up to 5kb or 8kb. Makes me wonder if taking a union of overlapping peaks is a good idea ? Are promoter regions really that long or Im lumping them all and missing out on information..

ADD REPLYlink written 7.0 years ago by aditi.qamra270
1

There might be different cases why the peak looks broad

  1. it might be the promoter of an upstream gene
  2. unannotated promoters
  3. a small (noise) peak joining the tail of a normal peak making it look peak
  4. If you center your peaks at TSS and make a union, it will take into consideration the variable widths of the peaks, as for most of the peaks, 3KB upstream would come clean and for some not, so with the union you will see that, highest in the centre and then a decreasing gradient.

Cheers

ADD REPLYlink modified 7.0 years ago • written 7.0 years ago by Sukhi Singh10k

Do you know why H3K3me3 shows a small peak before the TSS and a more sharper peak after TSS? 

ADD REPLYlink written 5.4 years ago by tonja.r490
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 2059 users visited in the last hour
_